Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: Effect of 3α-THP on human GB cell migration. ( a ) Representative images of the scratch area from U251 cells treated with vehicle (V, ethanol 0.1% in the medium), progesterone (P4; 10 nM), or allopregnanolone (3α-THP; 10, 100 nM and 1 µM). Percentage migration graphs of ( b ) U251, ( c ) U87, and ( d ) LN229 human GB cell lines. Each point represents the mean ± SEM. U251 (Graph b): # p < 0.05 for P4 and 3α-THP 100 nM vs. V; * p < 0.05 for P4 and all concentrations of 3α-THP vs. V. U87 (Graph c): * p < 0.05 for 3α-THP 10 nM, 100 nM and 1 µM vs. V. LN229 (Graph d): + p < 0.05 3α-THP 10 nM and 1 µM vs. V; * p < 0.05 for 3α-THP 100 nM vs. all other treatments. n = 3 for U251 and U87 cells; n = 4 for LN229 cell line.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques: Migration

Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: Effect of 3α-THP on the invasion of human GB cell lines. Cells were treated with vehicle (V; ethanol, 0.1% in the medium), P4 (10 nM), and 3α-THP (100 nM) for 24 h. Representative images of ( a ) U251; ( c ) U87, and ( e ) LN229 invasion assays. All photographs were taken at 10× augment. Graphs of the invading cells number per field: ( b ) U251; ( d ) U87; and ( f ) LN229 cell lines. Each column represents the mean ± SEM. n = 3 for all cell lines evaluated; * p < 0.05 vs. V.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: AKR1C1-4 are differentially expressed in GB cells. The expression of AKR1C1-4 was detected in a total protein extract from normal human astrocytes (HA) and different human GB cell lines. ( a ) Representative Western blots of AKR1C1-4 and α-Tubulin, which was used as a loading control. ( b ) Densitometric analysis graph. Each column represents the mean ± SEM. n = 3; * p < 0.05 U251, T98G, and LN229 vs. HA and U87 cell lines.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: Effect of 3α-THP and the pharmacological inhibitor of c-SRC PP2 on U251 and U87 cell invasion. ( a ) Representative images of U251 cell invasion assays. All photographs were taken at 10× augment. ( b ) Graph of the number of invading U251 cells per field. Each column represents the mean ± SEM., n = 4; * p < 0.05 3α-THP vs. V (EtOH, and DMSO); ** p < 0.005 3α-THP vs. 3α-THP + PP2. ( c ) Graph of the number of invading U87 cells per field. Each column represents the mean ± SEM., n = 4; * p < 0.05 3α-THP vs. all other treatments.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques:

Journal: Oncology Letters

Article Title: Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma

doi: 10.3892/ol.2020.11548

Figure Lengend Snippet: CHL1 expression level is correlated with PCNA expression levels in different grades of human gliomas, and downregulation of CHL1 induces glioma cell senescence. (A) Representative immunofluorescence staining of PCNA and CHL1 in CAN and human glioma tissues (graded I–II and II). Scale bars, 50 µm. (B) Correlation between CHL1 and PCNA expression levels (n=23, 18, 85, 27 and 11 for I, I–II, II, III and IV grades, respectively). (C) Immunofluorescence staining of CHL1 and PCNA in U-87 MG cells treated with either a control siRNA or an siRNA targeting CHL1 for 48 h. Downregulation of CHL1 resulted in the reduction of PCNA expression levels. Scale bars, 25 µm. (D) Enhanced staining of senescence-associated β-galactosidase was observed in cells with CHL1 knockdown, suggesting that CHL1 may function by delaying the senescence of glioblastoma cells. CHL1, cell adhesion molecule L1 like; PCNA, proliferation cell nuclear antigen; si, small interfering; con, control.

Article Snippet: The human glioblastoma U-87 MG cell line (cat. no. CL-0238; unknown origin) and the human glioma U251 cell line (cat. no. CL-0237) were purchased from Procell Life Science and Technology Co., Ltd.

Techniques: Expressing, Immunofluorescence, Staining, Control, Knockdown

Journal: Oncology Letters

Article Title: Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma

doi: 10.3892/ol.2020.11548

Figure Lengend Snippet: Western blot analysis of CHL1 expression levels in cultured SHG-44 and U251 human glioma and U-87 MG human glioblastoma cells in response to 48 h of treatment with (A) NRG1α or (Β) NRG1β at doses ranging from 0 to 5.0 nM. (C) A total of 3 siRNAs targeting NRG1 reduced NRG1 and CHL1 expression levels. *P<0.05. CHL1, cell adhesion molecule L1 like; NRG1, neuregulin 1; si, small interfering; Mr, molecular mass.

Article Snippet: The human glioblastoma U-87 MG cell line (cat. no. CL-0238; unknown origin) and the human glioma U251 cell line (cat. no. CL-0237) were purchased from Procell Life Science and Technology Co., Ltd.

Techniques: Western Blot, Expressing, Cell Culture

Journal: Oncology Letters

Article Title: Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma

doi: 10.3892/ol.2020.11548

Figure Lengend Snippet: Expression levels and localization of CHL1 in U251 human glioma cells and U-87 MG human glioblastoma cells in response to treatment with 2.5 nM NRG1s. U251 and U-87 MG cells were treated with 2.5 nM NRG1α and NRG1β for 48 h. The cells were then fixed and immunostained for CHL1 (green) and p-Neu (red), and then counterstained with DAPI (blue). Representative immunofluorescence staining showing the effects of NRG1α and NRG1β on CHL1 expression levels and localization in (A) U251 and (B) U-87 MG cells., Scale bars 30 and 20 µm, respectively. CHL1, cell adhesion molecule L1 like; NRG1, neuregulin 1; NEU, ErbB2 receptor; p, phosphorylated.

Article Snippet: The human glioblastoma U-87 MG cell line (cat. no. CL-0238; unknown origin) and the human glioma U251 cell line (cat. no. CL-0237) were purchased from Procell Life Science and Technology Co., Ltd.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Oncology Letters

Article Title: Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma

doi: 10.3892/ol.2020.11548

Figure Lengend Snippet: Effects of NRG1s on the expression levels of p-ERK1/2 in U-87 MG cells. Representative images showing western blot analysis for p-ERK1/2 in SHG-44, U251 and U-87 MG human glioma/glioblastoma cells in response to (A) NRG1α or (B) NRG1β at doses ranging from 0 to 5.0 nM. NRG1, neuregulin 1; p, phosphorylated.

Article Snippet: The human glioblastoma U-87 MG cell line (cat. no. CL-0238; unknown origin) and the human glioma U251 cell line (cat. no. CL-0237) were purchased from Procell Life Science and Technology Co., Ltd.

Techniques: Expressing, Western Blot

Journal: Oncology Letters

Article Title: Anticancer effect of the oncolytic Newcastle disease virus harboring the PTEN gene on glioblastoma

doi: 10.3892/ol.2024.14752

Figure Lengend Snippet: PTEN restoration suppresses tumorigenesis in orthotopic GBM mouse through infection of rNDV. (A) Mice were orthotopically injected with U87-MG-Luc2 cells. A total of 40 days after tumor cell injection, the mice were infected with rNDV-PTEN or rNDV via i.v. injection. (B) Body weight of in vivo mouse GBM models. (C) Survival rate of orthotopic GBM mouse models following rNDV-PTEN, rNDV or PBS injections (n=5). (D) Bioluminescent images of luciferase activity. Bioluminescent images were taken with IVIS Lumina XR and analyzed using Living Image Software (n=5). (E) MRI in orthotopic GBM mouse models. Representative MRI image of orthotopic GBM mouse models, taken 50 days after tumor injection (rNDV-PTEN, rNDV or PBS was injected 5 times). These data are expressed as the fold change in expression compared with PBS injected mice. *P<0.05 vs. PBS-injected mice. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MRI, magnetic resonance imaging; i.v., intravenous.

Article Snippet: Human GBM cells, U87-MG (cat no. HTB-14; GBM of unknown origin), U87-MG-luc2 (cat. no. HTB-14-LUC2; GBM of unknown origin), T98G (cat. no. CRL-1690) and CCF-STTG1 (cat. no. CRL-1718), were purchased from American Type Culture Collection (ATCC).

Techniques: Infection, Injection, In Vivo, Luciferase, Activity Assay, Software, Expressing, Recombinant, Virus, Magnetic Resonance Imaging