Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: Effect of 3α-THP on human GB cell migration. ( a ) Representative images of the scratch area from U251 cells treated with vehicle (V, ethanol 0.1% in the medium), progesterone (P4; 10 nM), or allopregnanolone (3α-THP; 10, 100 nM and 1 µM). Percentage migration graphs of ( b ) U251, ( c ) U87, and ( d ) LN229 human GB cell lines. Each point represents the mean ± SEM. U251 (Graph b): # p < 0.05 for P4 and 3α-THP 100 nM vs. V; * p < 0.05 for P4 and all concentrations of 3α-THP vs. V. U87 (Graph c): * p < 0.05 for 3α-THP 10 nM, 100 nM and 1 µM vs. V. LN229 (Graph d): + p < 0.05 3α-THP 10 nM and 1 µM vs. V; * p < 0.05 for 3α-THP 100 nM vs. all other treatments. n = 3 for U251 and U87 cells; n = 4 for LN229 cell line.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques: Migration

Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: Effect of 3α-THP on the invasion of human GB cell lines. Cells were treated with vehicle (V; ethanol, 0.1% in the medium), P4 (10 nM), and 3α-THP (100 nM) for 24 h. Representative images of ( a ) U251; ( c ) U87, and ( e ) LN229 invasion assays. All photographs were taken at 10× augment. Graphs of the invading cells number per field: ( b ) U251; ( d ) U87; and ( f ) LN229 cell lines. Each column represents the mean ± SEM. n = 3 for all cell lines evaluated; * p < 0.05 vs. V.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: AKR1C1-4 are differentially expressed in GB cells. The expression of AKR1C1-4 was detected in a total protein extract from normal human astrocytes (HA) and different human GB cell lines. ( a ) Representative Western blots of AKR1C1-4 and α-Tubulin, which was used as a loading control. ( b ) Densitometric analysis graph. Each column represents the mean ± SEM. n = 3; * p < 0.05 U251, T98G, and LN229 vs. HA and U87 cell lines.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation

doi: 10.3390/ijms23094996

Figure Lengend Snippet: Effect of 3α-THP and the pharmacological inhibitor of c-SRC PP2 on U251 and U87 cell invasion. ( a ) Representative images of U251 cell invasion assays. All photographs were taken at 10× augment. ( b ) Graph of the number of invading U251 cells per field. Each column represents the mean ± SEM., n = 4; * p < 0.05 3α-THP vs. V (EtOH, and DMSO); ** p < 0.005 3α-THP vs. 3α-THP + PP2. ( c ) Graph of the number of invading U87 cells per field. Each column represents the mean ± SEM., n = 4; * p < 0.05 3α-THP vs. all other treatments.

Article Snippet: Human GB cell lines U251, LN229, T98G, and U87 (unknown origin) were purchased from ATCC.

Techniques:

Journal: Oncology Letters

Article Title: Anticancer effect of the oncolytic Newcastle disease virus harboring the PTEN gene on glioblastoma

doi: 10.3892/ol.2024.14752

Figure Lengend Snippet: PTEN restoration suppresses tumorigenesis in orthotopic GBM mouse through infection of rNDV. (A) Mice were orthotopically injected with U87-MG-Luc2 cells. A total of 40 days after tumor cell injection, the mice were infected with rNDV-PTEN or rNDV via i.v. injection. (B) Body weight of in vivo mouse GBM models. (C) Survival rate of orthotopic GBM mouse models following rNDV-PTEN, rNDV or PBS injections (n=5). (D) Bioluminescent images of luciferase activity. Bioluminescent images were taken with IVIS Lumina XR and analyzed using Living Image Software (n=5). (E) MRI in orthotopic GBM mouse models. Representative MRI image of orthotopic GBM mouse models, taken 50 days after tumor injection (rNDV-PTEN, rNDV or PBS was injected 5 times). These data are expressed as the fold change in expression compared with PBS injected mice. *P<0.05 vs. PBS-injected mice. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MRI, magnetic resonance imaging; i.v., intravenous.

Article Snippet: Human GBM cells, U87-MG (cat no. HTB-14; GBM of unknown origin), U87-MG-luc2 (cat. no. HTB-14-LUC2; GBM of unknown origin), T98G (cat. no. CRL-1690) and CCF-STTG1 (cat. no. CRL-1718), were purchased from American Type Culture Collection (ATCC).

Techniques: Infection, Injection, In Vivo, Luciferase, Activity Assay, Software, Expressing, Recombinant, Virus, Magnetic Resonance Imaging